From the editor, what do you know about vacuum centrifuge concentrators?

Vacuum centrifuge concentrator uses vacuum to reduce the boiling point of solvent, vacuum pump and/or cold trap to collect the evaporated vapor in time to remove the solvent, dry/concentrate the sample. Because of continuous vacuum pumping, the boiling point of the solvent will decrease under vacuum condition, and the sample temperature will also decrease, which can effectively ensure the sample activity. A certain centrifugal force will make the sample always precipitate at the bottom of the container, almost without any loss; It can also handle a lot of samples at the same time. Compared with nitrogen blowing and rotary steaming, Xiaobian feels that it is much stronger in sample handling capacity, ensuring sample activity and concentration efficiency. After user trial, it is very good, and it is estimated that few people know it. Before many in the propaganda emphasis on lyophilization, coupled with the national standard recommended rotary evaporation and nitrogen blowing instrument and other factors, so vacuum centrifugation concentration is low-key.

Nitrogen blowing is to concentrate some volatile solvents such as low boiling point ethanol under normal temperature and pressure. Vacuum centrifugal concentrator can concentrate solvents with medium, high and low boiling points by vacuum control. From my own understanding of this instrument, I think it is a good instrument, which can not only ensure sample activity, but also several times faster than lyophilization. It is much larger than rotary steam and nitrogen blowing instrument to deal with samples. And also trust the professional manufacturer's software and data, to optimize the experimental scheme is very helpful!!

Vacuum centrifugal concentration meter on the design to use "control vacuum" key characteristics, there is not much to limit sample weight, which is better than a feature of the advantages of freeze drying, freeze drying need sample thickness is 1 cm, otherwise the lyophilization process will be slower), cheng fang sample container is actually placed on centrifugal rotor of the centrifugal vacuum concentration meter host, The rotor has a certain tilt Angle, so taking into account that the liquid surface may flow out of a certain slope after the tilt, as long as the load is not too full, it can be loaded about 95%. Concentration meter is not the same as the size, the distribution of vacuum pump is not the same, any cold trap, host is not the same as the heating temperature, different set vacuum will affect the efficiency of enrichment, for example, use common enrichment apparatus enrichment nucleic acid in ethanol, enrichment, 70 ml about half an hour, if large infrared heating machine, also is a few minutes.

From the user's point of view, is to clearly know the experimental progress, panel display parameters, it is convenient to optimize and adjust the experimental scheme;

Vacuum concentration is the key to the control of the vacuum, ---- with a good pump, no control function on the pump pumping, the temperature is too low to lead to the sample freezing evaporation into sublimation, into freeze-dried, then the rate is absolutely slow down; With a pump, high temperature is easy to cause the samples, so the vacuum control absolutely necessary, in view of the instrument panel display data to adjust the Settings again vacuum and heating temperature, the sample handling up absolutely effective, so I'm more inclined to customize pump to compose a concentrated system, rather than buy don't brand enrichment system of the built-in pump, Built-in pump system although initially selected instrument can save snacks, but do experiments have a situation in the future, in what isn't very convenient for the pump, and a few manufacturer of vacuum centrifugal enrichment system of vacuum control function or not, or is not standard, or according to real data is not comprehensive, then can keep on the centrifugal speed, rotor on make up, looked very tough to get parameters, But it ignores the importance of a substantial vacuum.